Co-Localization of Osteoclasts Differentiation Markers in an Implant Model in Diabetic Mice

Authors

Diego Adobbati, The University of Texas Rio Grande ValleyFollow
Deborah Rocha Seixas, The University of Texas Rio Grande Valley
Danieli Rodrigues, University of Texas at Dallas
Claudia C. Biguetti, The University of Texas Rio Grande ValleyFollow

Document Type

Poster

Publication Date

Fall 7-26-2024

Abstract

Introduction and Hypothesis: Diabetes can impair implant osseointegration and impact implant functionality. While significant research has focused on osteogenesis during the osseointegration, little attention has been given to pre-osteoclast differentiation. We hypothesize that diabetes increases pre-osteoclast activity potentially leading to implant failure. This study aims to identify and localize osteoclast markers, specifically DC-STAMP (Dendritic Cell-Specific Transmembrane Protein) and NFATc2 (Nuclear Factor of Activated T-cells, cytoplasmic 2), in diabetic (D) and non-diabetic (ND) mice.

Methodology: Twenty-four 16-week-old male 129 Sv mice (12 diabetic and 12 non-diabetic) underwent implantation with a 0.5x1.5 mm commercially pure titanium screw in the right maxillary bone. Diabetes was induced in mice using a high-fat diet followed by two consecutive streptozotocin injections (100mg/kg) one week prior to surgery. At 7 and 21 days post-implantation, six mice from each group were euthanized, and samples were collected for immunofluorescence analysis targeting DC-STAMP (TRITC) and NFATc2 (FITC), with DAPI blue staining for nuclei.

Results: The D group exhibited signs of inflammation and incomplete epithelization, contrasting with the ND group, which showed signs of successful clinical healing. NFATc2 and DC-STAMP primarily co-localized in multinucleated cells, indicative of osteoclasts, in both groups. However, the intensity of both markers in ND group decreased significantly at 21 days, primarily found in remodeling trabeculae. Conversely, D-group from the same timeframe showed a widespread presence of co-localized DC-STAMP and NFATc2 cells, particularly in areas of implant failure.

Discussion and Conclusion: This study demonstrates that diabetes negatively impacts the osseointegration of titanium implants through the modulation of osteoclast differentiation and activity.

Recommended Citation

Adobbati, D., Rocha Seixas, D., Rodrigues, D. C., & Biguetti, C. C. (2025). Co-localization of osteoclast differentiation markers in an implant model in diabetic mice [Poster]. Regenerative Medicine Research Laboratory, The University of Texas Rio Grande Valley.