A Model Study to Evaluate Osseointegration and Fracture Healing Following Open Reduction and Internal Fixation (ORIF) in Diabetic Lewis Rats

Authors

Alexandra Arteaga, University of Texas at Dallas
Claudia Biguetti, The University of Texas Rio Grande ValleyFollow
Bhuvana Lakkasetter Chandrashekar, University of Texas at Dallas
Jimena Mora, University of Texas at Dallas
Adeena Qureshi, University of Texas at Dallas
Evelin Rios, University of Texas at Dallas
Javier La Fontaine, The University of Texas Rio Grande Valley
Danieli C. Rodrigues, University of Texas at Dallas

Document Type

Article

Publication Date

5-9-2023

Abstract

There is a higher risk of implant osseointegration failure after open reduction and internal fixation (ORIF) in patients with diabetes due to increased inflammatory conditions, associated metallic corrosion and infection. While it is possible to avoid elective osseous surgery in patients with diabetes, it may not be the case in nonelective cases, such as ORIF ankle fractures. A total of 30 male Lewis rats (12-15 weeks old) were distributed into diabetic (D) and nondiabetic (ND) groups. Fracture healing and osseointegration were evaluated at 2-, 10-, and 21-day time points. Microtomographic and histological analysis depicted distinct differences in fracture healing and osseointegration between D and ND animals. Immunohistochemical analysis exhibited elevated proliferation (PCNA) and osteogenic (Runx2) cells for ND animals, while HMGB1 (inflammatory marker) was elevated for D animals during healing. Bone resorption marker CTX-1 was elevated in the plasma of D animals at 2 days, while bone formation marker P1NP was higher for ND animals at 10 days. Overall, this model resulted in delayed implant osseointegration and fracture healing in diabetic animals, highlighting the importance of developing new biomaterials or implant coatings that can improve bone healing outcomes in this patient population.

Comments

Copyright © 2023 The Authors.

Recommended Citation

Arteaga, A., Biguetti, C. C., Chandrashekar, B. L., Mora, J., Qureshi, A., Rios, E., La Fontaine, J., & Rodrigues, D. C. (2023). A Model Study to Evaluate Osseointegration and Fracture Healing Following Open Reduction and Internal Fixation (ORIF) in Diabetic Lewis Rats. The Journal of foot and ankle surgery : official publication of the American College of Foot and Ankle Surgeons, S1067-2516(23)00115-1. Advance online publication. https://doi.org/10.1053/j.jfas.2023.04.011

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publication Title

The Journal of foot and ankle surgery : official publication of the American College of Foot and Ankle Surgeons

Academic Level

faculty

DOI

https://doi.org/10.1053/j.jfas.2023.04.011