Document Type
Article
Publication Date
9-1985
Abstract
Effects of pH, incubation time, nitrogen source and carbohydrate source upon production of carboxymethyl cellulase by Aspergillus candidus grown in liquid medium were studied. Maximal cellulase activity per unit of protein occurred in culture fluids with a pH of 5.5, after 10 days of cultivation of the fungus. Ammonium nitrate supported the highest production of the enzyme. However, in the nitrogen-source test, highest accumulation of extracellular protein was found in fluids from cultures with urea as the nitrogen source. Cotton fibers produced the greatest gain in cellulase activity when the carbohydrate source was incremented from 0.5 to 1.0 percent. The highest cellulase activity was measured in fluids whose carbohydrate source was carboxymethyl cellulase (CMC). In the carbohydrate-source test, highest accumulation of extracellular protein was found in fluids from cultures with powdered cellulose as the carbohydrate source, at 1.0 percent concentration.
Recommended Citation
Ortega, Jacobo, and Ernest Baca. “Factors Influencing Cellulolytic Activity of the Soil Fungus, Aspergillus Candidus.” Texas Journal of Science 37, no. 2 & 3 (September 1985): 245–52.
First Page
245
Last Page
252
Publication Title
Texas Journal of Science
Comments
Copyright The Texas Journal of Science.