DNA adenine methylase is essential for viability and plays a role in the pathogenesis of Yersinia pseudotuberculosis and Vibrio cholerae

Authors

S. M. Julio, University of California, Santa Barbara
D. M. Heithoff, University of California, Santa Barbara
Daniele Provenzano, The University of Texas Rio Grande ValleyFollow
K. E. Klose, University of California, Santa Barbara
R. L. Sinsheimer, University of California, Santa Barbara
D. A. Low, University of California, Santa Barbara
M. J. Mahan, University of California, Santa Barbara

Document Type

Article

Publication Date

12-5-2001

Abstract

Salmonella strains that lack or overproduce DNA adenine methylase (Dam) elicit a protective immune response to different Salmonella species. To generate vaccines against other bacterial pathogens, the dam genes of Yersinia pseudotuberculosis and Vibrio cholerae were disrupted but found to be essential for viability. Over-production of Dam significantly attenuated the virulence of these two pathogens, leading to, in Yersinia, the ectopic secretion of virulence proteins (Yersinia outer proteins) and a fully protective immune response in vaccinated hosts. Dysregulation of Dam activity may provide a means for the development of vaccines against varied bacterial pathogens.

Comments

Copyright © 2001, American Society for Microbiology

Recommended Citation

Julio, Steven M., Douglas M. Heithoff, Daniele Provenzano, Karl E. Klose, Robert L. Sinsheimer, David A. Low, and Michael J. Mahan. "DNA adenine methylase is essential for viability and plays a role in the pathogenesis of Yersinia pseudotuberculosis and Vibrio cholerae." Infection and immunity 69, no. 12 (2001): 7610-7615. https://doi.org/10.1128/iai.69.12.7610-7615.2001

First Page

7610

Last Page

7615

Publication Title

Infection and Immunity

DOI

10.1128/IAI.69.12.7610-7615.2001