Theses and Dissertations

Date of Award

8-2022

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biochemistry and Molecular Biology

First Advisor

Dr. Manish Tripathi

Second Advisor

Dr. Bilal Hafeez

Third Advisor

Dr. Murali Yallapu

Abstract

Liver cancer is the fifth leading cause of mortality attributed to cancer. Among various histological types, hepatocellular carcinoma (HCC) is the most prevalent form of liver cancer. There is a need to identify new biomarkers to help the physician, and allow them to decide what may be the most effective treatment for the patient. POTE-2 is one of the 14 paralogs apart of the ancestral POTE gene family. POTE-2 has been shown to be upregulated in non-small cell lung cancer and colorectal cancer, and is associated with increased migration, invasion, tumor size and poor survival. Evaluation of the Cancer Genome Atlas for POTE-2 in liver cancer showed increased expression in malignant vs tumor and expression correlated with increased stage and grade. This was further validated through a liver cDNA and tissue microarray. Gain of function studies showed increased phosphorylation of major kinases and increased cellular invasion. The findings indicate that POTE-2 expression is increased in cancer, is associated with advanced cancer, and could play a significant role in cancer progression.

Comments

Copyright 2022 Samantha Lopez. All Rights Reserved.

https://go.openathens.net/redirector/utrgv.edu?url=https://www.proquest.com/dissertations-theses/role-pote-ankyrin-domain-family-member-2-liver/docview/2741095325/se-2?accountid=7119

Available for download on Friday, November 29, 2024

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