Theses and Dissertations

Date of Award

5-2023

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Agricultural, Environmental, and Sustainability Sciences

First Advisor

Dr. MD Saydur Rahman

Second Advisor

Dr. Amit Raysoni

Third Advisor

Dr. Lucia Carreon Martinez

Abstract

Aquatic organisms are frequently exposed to various environmental stressors. In this study, we observed the short-term (1-week) combined dose-dependent effect of Roundup, a glyphosate-based herbicide, and high temperature (30°C) on the morphology of gills and digestive glands, expression of heat shock protein-70 (HSP70), dinitrophenyl protein (DNP), nitrotyrosine protein (NTP), antioxidant enzymes such as catalase (CAT) and superoxidase dismutase (SOD) in tissues of American oyster (Crassostrea virginica). Histological analyses showed an increase in mucous production in the gills and digestive glands, hemocyte aggregation in the connective tissues as well as structural change of the lumen in the digestive glands of oysters co-exposed to high temperature and Roundup. Alternation in the expression of HSP70, NTP, DNP, CAT, and SOD was also observed through immunohistochemical analyses. Collectively, these results suggest that combined exposure to high temperature and Roundup cause oxidative/nitrative stress through overproduction of ROS/RNS which alter the prooxidant-antioxidant status in marine mollusks.

Comments

Copyright 2023 Afsana Chowdhury. All Rights Reserved.

https://go.openathens.net/redirector/utrgv.edu?url=https://www.proquest.com/dissertations-theses/effect-elevated-temperature-roundup-exposure-on/docview/2842690030/se-2?accountid=7119

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