Theses and Dissertations

Date of Award

5-2024

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biochemistry and Molecular Biology

First Advisor

Upal Roy

Second Advisor

Nirakar Sahoo

Third Advisor

Hansapani Rodrigo

Abstract

The advent of cART has revolutionized the management of HIV-1 infection and saved the lives of millions of people worldwide. Yet, HAND continues to be clinically relevant with aging people with HIV-1 (PWH). The underlying mechanism of HAND remains poorly understood. In this regard. Signal transducer and activator of transcription 1(STAT1) and IFIT3 genes have been shown to induce neuroinflammation and neuronal cell death. However, the role of IFIT3 in HAND pathology has not been well established. Therefore, the present work investigated the significant role of IFIT3 gene in development of HAND. In the current study, we used an in-vitro model to expose the neuroblastoma cell-line SH-SY5Y with clinically relevant cARTs drug and HIV Tat protein to observe the STAT1 and IFIT3 genes dysregulation. Furthermore, the study also investigates the STAT1 and IFIT3 protein dysregulation through immunocytochemistry and Western Blot assay. Overall observation indicated that HIV-Tat protein upregulated gene expression whereas with the cART exposure there was a downregulation of STAT1 and IFIT3 genes. The current study has established IFIT3 as a key molecular marker to develop a therapeutic target for the treatment of HAND.

Comments

Copyright 2024 Ranjit Kumar Das.

https://go.openathens.net/redirector/utrgv.edu?url=https://www.proquest.com/pqdtglobal1/dissertations-theses/role-interferon-induced-protein-with/docview/3085296397/sem-2?accountid=7119

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