Local Delivery of Mesenchymal Stem Cells Expressing Osteogenesis-Inducing MicroRNA for Bone Tissue Engineering Applications

Author

Marco Antonio Arriaga, The University of Texas Rio Grande Valley

Date of Award

12-2018

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Dr. Sue Anne Chew

Second Advisor

Dr. Boris Ermolinsky

Third Advisor

Dr. Daniele Provenzano

Abstract

Bone is capable of regenerating autonomously when the injury is small (< 2.5 cm), however, large (> 2.5 cm) or insufficiently vascularized defects require intervention to repair. The overall goal of this project is to contribute towards the development of novel strategies to aid bone tissue regeneration by delivering mesenchymal stem cells expressing microRNAs that induce expression of the runt-related transcription factor 2 (RUNX2). It was determined that porous polycaprolactone (PCL) scaffolds fabricated to have a higher surface area to volume ratio (SVR) (> 80) compared to lower SVR (< 80) were hydrolyzed at a higher rate. Scaffolds fabricated with 80 vol. %/300–500 µm porogen were optimal for cell colonization as higher absorbance of metabolized MTT was detected compared to other scaffold types. From the subset of 5 miRNAs tested, miRNA-26a, miRNA-196 and miRNA-218 demonstrated higher upregulation of RUNX2 gene expression and calcium mineralization and thus, are promising candidates for bone tissue regeneration.

Comments

Copyright 2018 Marco Antonio Arriaga. All Rights Reserved..

https://www.proquest.com/dissertations-theses/local-delivery-mesenchymal-stem-cells-expressing/docview/2177369803/se-2?accountid=7119

Recommended Citation

Arriaga, Marco Antonio, "Local Delivery of Mesenchymal Stem Cells Expressing Osteogenesis-Inducing MicroRNA for Bone Tissue Engineering Applications" (2018). Theses and Dissertations. 156.
https://scholarworks.utrgv.edu/etd/156