Label-Free Laser-Induced Graphene-Based Electrochemical Biosensor Modified With Graphene-Conductive Polymer Ink Coating for Detection of Alpha-Fetoprotein and 17β – Estradiol

Author

Ridma Tabassum, The University of Texas Rio Grande Valley

Date of Award

8-1-2024

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Electrical Engineering

First Advisor

Nazmul Islam

Second Advisor

Ali Ashraf

Third Advisor

Paul Choi

Abstract

In this study, a novel electrochemical biosensor was proposed based on inducing graphene formation on polyimide substrate via laser engraving. Graphene polyaniline (G-PANI) conductive ink was synthesized by planetary mixing and applied to the working zone of the developed sensor to effectively enhance the electrical signals. The laser-induced graphene (LIG) sensor was used to detect alpha-fetoprotein (AFP) and 17β-Estradiol in the phosphate buffer saline (PBS) buffer and human serum. The electrochemical performance of the biosensor in determining these biomarkers was investigated by differential pulse voltammetry (DPV) and chronoamperometry (CA). In a buffer environment, alpha-fetoprotein (AFP) and 17β-Estradiol detection range were 4-400 ng/mL and 20-400 pg/mL respectively. The electrodes showed lower detection limits (LODs) of 1.15 ng/mL and 0.96 pg/mL for AFP and estrogen, respectively, with an excellent linear range (R2= 0.98 and 0.99). In addition, the designed sensor was able to detect these two types of biomarkers in human serum successfully. The proposed sensor exhibited excellent reproducibility, repeatability, and good stability.

Comments

Copyright 2024 Ridma Tabassum. https://proquest.com/docview/3115398615

Recommended Citation

Tabassum, Ridma, "Label-Free Laser-Induced Graphene-Based Electrochemical Biosensor Modified With Graphene-Conductive Polymer Ink Coating for Detection of Alpha-Fetoprotein and 17β – Estradiol" (2024). Theses and Dissertations. 1573.
https://scholarworks.utrgv.edu/etd/1573