Optical method for high magnification imaging and video recording of live cells at sub-micron resolution

Author

Jaime E. Romo Jr., The University of Texas Rio Grande Valley

Date of Award

12-2016

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Physics

First Advisor

Dr. Natalia V. Guevara

Second Advisor

Dr. Juan Guevara Jr.

Third Advisor

Dr. Ahmed Touhami

Abstract

Optical microscopy, the most common technique for viewing living microorganisms, is limited in resolution by Abbe’s criterion. Recent microscopy techniques focus on circumnavigating the light diffraction limit by using different methods to obtain the topography of the sample. Systems like the AFM and SEM provide images with fields of view in the nanometer range with high resolvable detail, however these techniques are expensive, and limited in their ability to document live cells. The Dino-Lite digital microscope coupled with the Zeiss Axiovert 25 CFL microscope delivers a cost-effective method for recording live cells. Fields of view ranging from 8 microns to 300 microns with fair resolution provide a reliable method for discovering native cell structures at the nanoscale.

In this report, cultured HeLa cells are recorded using different optical configurations resulting in documentation of cell dynamics at high magnification and resolution.

Comments

Copyright 2016 Jaime E. Romo Jr. All Rights Reserved.

https://www.proquest.com/dissertations-theses/optical-method-high-magnification-imaging-video/docview/1878202743/se-2?accountid=7119

Recommended Citation

Romo, Jaime E. Jr., "Optical method for high magnification imaging and video recording of live cells at sub-micron resolution" (2016). Theses and Dissertations. 181.
https://scholarworks.utrgv.edu/etd/181