Theses and Dissertations

Date of Award

5-2018

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Mechanical Engineering

First Advisor

Dr. Karen Lozano

Second Advisor

Dr. Robert Gilkerson

Third Advisor

Dr. Rogelio Benitez

Abstract

This thesis presents the successful development of biocompatible Polyvinyl Butyral (PVB), PVB/Polylysine and PVB/Tannic Acid (TA)/Polylysine fibers from an ethanol solution, Polyhydroxybutyrate (PHB), PHB/Polylysine, PHB/TA/Polylysine fibers from a chloroform solution and Chitosan (CH)/Pullulan (PL)/TA and CH/PL/TA/Polylysine fibers from an aqueous solution. The fibers were mass produced utilizing the Forcespinning® (FS) technology. The morphology of the fibers was characterized using a scanning electron microscope (SEM) and the fibers average diameter was calculated. The thermal properties of the fibers were characterized using a thermogravimetric analyzer (TGA) and a differential scanning calorimeter (DSC). The antibacterial activity of the fibers was assessed using against Escherichia coli. The biocompatibility of the fibers was studied by culturing NIH 3T3 Mouse Embryonic Fibroblast cells in the fibers. These fibers provide the cells with a 3D environment mimicking the extracellular matrix (ECM) in the skin which favors cell adherence and attachment, thus favoring skin wound healing.

Comments

Copyright 2018 Astrid Michelle Rodriguez Negron. All Rights Reserved.

https://www.proquest.com/dissertations-theses/fibers-skin-regeneration/docview/2097246097/se-2?accountid=7119

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