Theses and Dissertations
Date of Award
12-2018
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biology
First Advisor
Dr. Michael Wayne Persans
Second Advisor
Dr. Megan Keniry
Third Advisor
Dr. Teresa Patricia Feria
Abstract
Superoxide Dismutase enzyme activity was analyzed in transgenic Arabidopsis thaliana, a non-accumulator weed plant species of the Brassicaceae family, that was transformed with Streptanthus farnsworthianus Metal Tolerance Protein1 (SfMTP1) or A. thaliana Metal Tolerance Protein 1 (AtMTP1 ). The transgenic A. thaliana was exposed to a 12 day treatment of 100 ?M Nickel Acetate in 1/10 X Hoaglands solution in hydroponics. Analysis of Super Oxide Dismutase activity determined that transgenic A. thaliana transformed with the AtMTP1 cDNA and treated with 100 µM Nickel showed a significant increase in Super Oxide Dismutase activity compared to A. thaliana transformed with the empty vector pKYLX71. Transgenic A. thaliana transformed with the SfMTP1 cDNA demonstrated a significant increase in Super Oxide Dismutase activity compared to A. thaliana transformed with pKYLX71. A. thaliana transgenic plants transformed with the AtMTP1 cDNA had higher/lower overall SOD activity compared to A. thaliana transformed with SFMTP1 cDNA.
Recommended Citation
Urbina Fernandez, Selina, "Arabidopsis Thaliana Transformed with Streptanthus farnsworthianus Metal Tolerance Protein 1 (SfMTP1) or the Native A. thaliana Metal Tolerance Protein 1 ( AtMTP1) cDNA and the Effects on SOD Enzyme Activity upon Nickel Treatment" (2018). Theses and Dissertations. 550.
https://scholarworks.utrgv.edu/etd/550
Comments
Copyright 2018 Selina Urbina Fernandez. All Rights Reserved.
https://go.openathens.net/redirector/utrgv.edu?url=https://www.proquest.com/dissertations-theses/arabidopsis-thaliana-transformed-with-i/docview/2177746044/se-2?accountid=7119