Role of Caspase-8 on Human Retinal Microvasculature Endothelial Cells (HRMEC) Migration in Hyperglycemia

Author

Paola A. Campano, The University of Texas Rio Grande Valley

Date of Award

8-2021

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Dr. Andrew Tsin

Second Advisor

Dr. Megan Keniry

Third Advisor

Dr. Michael Persans

Abstract

Pathological angiogenesis is the hallmark of proliferative diabetic retinopathy that results from the advanced stage of Diabetic Retinopathy (DR). In response to prolonged tissue injury, retinal microvascular endothelial cells form new blood vessels to ensure the supply of oxygen and nutrients to the eye; however, progressive fibrovascular proliferation can eventually lead to retinal detachment and blindness. Caspase-8, the responsible protease for apoptosis has also been shown to modulate pathological and developmental angiogenesis but whether it exerts this role in a hyperglycemic environment has not yet been explored. Here we demonstrate that Caspase-8 decreased cell viability and was upregulated in hyperglycemic HRMEC. In addition, Caspase-8 K.D. resulted in inhibition of cell migration. Taken together, our data suggest that Caspase-8 plays a role that is involved in cell migration in HRMEC.

Comments

Copyright 2021 Paola Campano. All Rights Reserved.

https://go.openathens.net/redirector/utrgv.edu?url=https://www.proquest.com/dissertations-theses/role-caspase-8-on-human-retinal-microvasculature/docview/2600467033/se-2?accountid=7119

Recommended Citation

Campano, Paola A., "Role of Caspase-8 on Human Retinal Microvasculature Endothelial Cells (HRMEC) Migration in Hyperglycemia" (2021). Theses and Dissertations. 836.
https://scholarworks.utrgv.edu/etd/836