Theses and Dissertations

Date of Award

8-2022

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Andrew Tsin

Second Advisor

Megan Keniry

Third Advisor

Robert Gilkerson

Abstract

Diabetic retinopathy is an ocular disease which appears in patients who experience progression of diabetes mellitus over a continuous period. Oftentimes, patients go undiagnosed due to the fact that there is no way to determine when the exact moment a patient develops diabetic retinopathy. Ophthalmologists and other eye specialists diagnose a patient with diabetic retinopathy once the patient begins to show progressed symptoms of the disease. Various experiments have been previously performed to increase our knowledge of the connection of diabetic retinopathy and early biomarkers of the disease. Several studies have determined the effects of diabetic retinopathy and apoptosis with cytochrome c presence using bovine retinal cells and rat models. The purpose of this experiment is to understand, analyze and quantify the effects of diabetic conditions on cytochrome c presence in the mitochondria of human retinal pericyte cells by using TUNEL, heme staining and Western Blot methods. In doing so, I will determine whether cytochrome c levels could serve as potential biomarkers in the early detection of diabetic retinopathy.

Comments

Copyright 2022 Ileana G. Villarreal. All Rights Reserved.

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