Theses and Dissertations
Date of Award
12-2020
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Ocean, Coastal, and Earth Sciences
First Advisor
Dr. MD Saydur Rahman
Second Advisor
Dr. Amit Raysoni
Third Advisor
Dr. Lucia B. Carreon Martinez
Abstract
Global temperature increases due to anthropogenic activities. The effects of rising temperature are well documented in aquatic organisms. The American oyster is an ideal shellfish species to study on global warming and oxidative DNA damage. In this study, We observed the effects of high temperature on heat shock protein-70 (HSP70), 8-hydroxy-2’-deoxyguanosine (8-OHdG), ssDNA, dsDNA, caspase-3 (CASP3), BAX and γH2AX, a molecular marker of DNA damage, protein expressions in gills of oysters. Immunohistochemical results showed that elevated temperatures (28 and 32°C) significantly increased HSP70, 8-OHdG, dsDNA, γH2AX, BAX and CASP3 protein expressions in gills of oysters compare to control (24°C). In situ TUNEL assay showed a significant increase in apoptotic cells in gills. Extrapallial fluid (EPF) glucose level also increased; however, EPF protein concentration decreased in heat exposure oysters. Collectively, these results suggest that heat shock driven oxidative stress induces DNA damage which may lead to decreased physiological functions in oysters.
Recommended Citation
Rahman, Md Faizur, "Effects of Elevated Temperature on 8-Hydroxy-2'-Deoxyguanosine Expression, DNA Damage and Cellular Apoptosis in the American Oyster (Crassostrea virginica)" (2020). Theses and Dissertations. 746.
https://scholarworks.utrgv.edu/etd/746
Comments
Copyright 2020 Md Faizur Rahman. All Rights Reserved.
https://go.openathens.net/redirector/utrgv.edu?url=https://www.proquest.com/dissertations-theses/effects-elevated-temperature-on-8-hydroxy-2/docview/2554332456/se-2?accountid=7119