Theses and Dissertations

Date of Award


Document Type


Degree Name

Master of Science (MS)


Ocean, Coastal, and Earth Sciences

First Advisor

Dr. MD Saydur Rahman

Second Advisor

Dr. Amit Raysoni

Third Advisor

Dr. Lucia B. Carreon Martinez


Global temperature increases due to anthropogenic activities. The effects of rising temperature are well documented in aquatic organisms. The American oyster is an ideal shellfish species to study on global warming and oxidative DNA damage. In this study, We observed the effects of high temperature on heat shock protein-70 (HSP70), 8-hydroxy-2’-deoxyguanosine (8-OHdG), ssDNA, dsDNA, caspase-3 (CASP3), BAX and γH2AX, a molecular marker of DNA damage, protein expressions in gills of oysters. Immunohistochemical results showed that elevated temperatures (28 and 32°C) significantly increased HSP70, 8-OHdG, dsDNA, γH2AX, BAX and CASP3 protein expressions in gills of oysters compare to control (24°C). In situ TUNEL assay showed a significant increase in apoptotic cells in gills. Extrapallial fluid (EPF) glucose level also increased; however, EPF protein concentration decreased in heat exposure oysters. Collectively, these results suggest that heat shock driven oxidative stress induces DNA damage which may lead to decreased physiological functions in oysters.


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